Beneficial effects of formononetin in type 2 diabetic rats

[Speaker] Manisha J. Oza:1,2
[Co-author] Yogesh A. Kulkarni:1
1:Shobhaben Pratapbhai Patel School of Pharmacy & Technology Mangement, SVKM's NMIMS, India, 2:SVKM's Dr. Bhanuben Nanavati College of Pharmacy, Mumbai, India

Type 2 diabetes is a serious metabolic disorder which results from defective insulin secretion and action. Recently reported data by Centers for Disease Control and Prevention showed that 90 to 95 % of the total reported cases of diabetes are of type 2 diabetes. Novel therapeutic strategies are necessary to control hyperglycemia and to improve quality of life in the patient suffering from diabetes. Flavonoids have shown significant effect in diabetic condition. Formononetin is an isoflavone type of flavonoids found in legume plants. The present study was designed to evaluate the effect of formononetin in the treatment of high fat diet and low dose streptozotocin induced type 2 diabetes in Sprague Dawley rats.
After acclimatization of animals for one week, type 2 diabetes mellitus was induced in Sprague Dawley rats by feeding the rats with high fat diet for 2 weeks and administration of 40 mg/kg of streptozotocin intraperitonially. The plasma glucose was measured one week after STZ administration. The animals with plasma glucose level greater than 16.7 mmol/L was considered diabetic and selected for further study. Diabetic animals were treated with formononetin at the dose of 10, 20 and 40 mg/kg of body weight for 28 days. Various biochemical parameters like plasma glucose, cholesterol, HDL, LDL, triglycerides and glycohaemoglobin were estimated. OGTT, estimation of oxidative stress parameters and Histopathological studies were done.
The outcome of the study showed that treatment with the formononetin significantly reduced the elevated level of plasma glucose (p < 0.001) in type 2 diabetic animals. Treatment with formononetin significantly increased plasma HDL level and reduced total cholesterol, total triglyceride and LDL concentration in type 2 diabetic rats. Formononetin supplementation resulted in significant (p<0.001) reduction of glycohaemoglobin, improved oral glucose tolerance and insulin sensitivity in diabetic rats. Pancreatic oxidative stress was significantly improved after treatment with formononetin in diabetic rats.
The result of the study showed that formononetin can be used to control hyperglycemia and to improve insulin sensitivity as well as lipid profile in type 2 diabetes mellitus.

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