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PO1-4-37

The cytokines profile in degenerative spine diseases: lumbar spondylolisthesis versus lumbar disc herniation

[Speaker] Juraj Sutovsky:1
[Co-author] Martin Benco:1, Michaela Kocmalova:2, Juraj Miklusica:3, Martina Sutovska:2
1:Neurosurgery Clinic, Jessenius Faculty of Medicine and Martin University Hospital, Comenius University, Kollarova 2, 036 01 Martin, Slovakia, 2:BioMed (Martin's Biomedical Center) and Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin's Biomedical Center (BioMed), Mala Hora, 11161 4D, 036 01 Martin, Slovakia, 3:Transplant and Vascular Surgery Clinic, Jessenius Faculty of Medicine and Martin University Hospital, Comenius University, Kollarova 2, 036 01 Martin, Slovakia

Background: Degenerative disorders of spine (DDS) are the most frequent reason of morbidity in adults. Commonly DDS includes degenerative disorders of intervertebral discs (IVDs), spinal stenosis and degenerative spondylolisthesis (DSL). There is increasing evidence about significant role of cytokines in DDS pathogenesis, symptomathology and progression, but their full meaning and protective levels remain still unknown.
Material and Methods: The aim of presented study was to provide quantitative and qualitative analysis of cytokine, chemokine and growth factors levels in individual parts of IVDs (annulus fibrosus (AF) and nucleus pulposus (NP)) of patients with DDS and in controls, healthy subjects during a multiorgan procurement procedure. The tissue samples were obtained during primary spinal surgery from 8 patients suffered from herniated lumbar IVDs and 9 patients with lower lumbar DSL. The subchondral bone tissue was collected from the adjacent facet joint (FJ) only in DSL group. Intact IVDs samples, used as a healthy control group, were obtained from 6 adult males during a multiorgan procurement procedure. Bio-Plex (Bio-Rad, USA) assay was used to measure concentrations of 27 different cytokines in tissue samples.
Results: The Bio-Plex assay revealed significant differences between the patients suffered from degenerated and herniated IVDs and from lumbar DSL in cytokines, chemokines and growth factor profiles suggested that pro-inflammatory changes of both NP and AF were dominated in herniated IVDs, whereas the same tissue of lumbar DSL patients exhibited much more complex changes consisted of inflammation (IL-6, IL-8, MCP-1, TNF-alpha), anti-inflammatory processes (IL-ra, IL-10) and connective tissue remodeling (PDGF-bb, IL-17, VEGF). Corresponded changes (inflammation and bone remodeling) were also evidenced in FJ bone of lumbar DSL samples.
Conclusion: The study supported the significant involvement of several cytokines, chemokines and growth factors in the pathogenesis of DDS. These cytokines should represent future potential targets for new biological treatment able to slow DDS progression as well as factor determining prognosis of DDS. Furthermore, the selected control samples and analytical methods used avoided any false changes in the cytokine levels due to secondary factors (e.g. death of donor and limited cytokine stability).


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