Program

PO1-4-21

Beta caryophyllene exerts its anti-allergic potency by inhibiting histamine release and pro-inflammatory markers in mast cells

[Speaker] Manash P Pathak:1,2
[Co-author] Pompy Patowary:1,2, Aparoop Das:2, Ps Raju:1, Pronobesh Chattopadhyay:1
1:Division of Pharmaceutical Technology, Defence Research Laboratory, Post Bag No. 2, Solmara, Tezpur-784001, Assam, India, 2:Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, 786 004, Assam, India

Background: Beta caryophyllene (BCP), a member of bicyclic sesquiterpene is found in large
amount in essential oils of many food and spice plants such as clove (Syzygium aromaticum),
cinnamon (Cinnamomum spp.), black pepper (Piper nigrum L.) and oregano (Origanum vulgare
L.). BCP exhibits potent anti inflammatory, anti clastogenic, anti cancer properties and is also
reported as an agonist for cannabinoid receptors. The aim of the present study was to elucidate
the anti allergic potential of BCP on mast cells and in vivo murine models as well as to study its
possible mechanisms of action.
Methods: The potency of BCP was screened by investigating the compound 48 80 induced
systemic allergic and immunoglobulin E (IgE) induced cutaneous allergic reaction in murine
model. Mast cell degranulation in rat peritoneal mast cells (RPMC) was evaluated followed by
fluorometric assessment of histamine release and intracellular calcium modulation. Furthermore,
pro inflammatory markers gene expression and production such as TNF and IL 6 as well as
expression of NF kB in mast cells were assessed by ELISA and western blotting.
Results: BCP attenuated compound 48 80 or IgE induced allergic reaction in murine model as
well as inhibited the mast cell degranulation and histamine release. BCP decreased the
expression of TNF and IL 6 in mast cells. Additionally, BCP inhibited the expression of NF kB
in mast cells. Finally, the inhibitory effect of BCP on the allergic reaction was found to be
similar with ketotifen fumarate, a standard mast cell stabilizer.
Conclusions: Our finding suggests that BCP inhibited the mast cell derived inflammatory
allergic reactions by blocking the expression of histamine release and pro inflammatory markers,
therefore, elucidating the underlying mechanisms of action. Furthermore, in vivo and in vitro
anti allergic effect of BCP suggests it to be a possible therapeutic agent for inflammatory allergic
conditions.
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