Protective effect of molecular hydrogen on Antimycin A-induced injury in H9c2 cardiomyocytes

[Speaker] Takahito Sonobe:1
[Co-author] Hideto Ariumi:1, Yuji Yoshiyama:1
1:Laboratory of Community Pharmacy, School of Pharmacy, Kitasato University, Japan

[Background] Periodontitis is common in adults and cardiovascular diseases (CVD) are the most common cause of adult death in the world. Opening of the infarct-related coronary artery is a valued therapeutic goal in acute myocardial infarction (AMI), however, cardiomyocytes continue to die during reperfusion. Acute oxidative stress induced by ischemia - reperfusion causes serious damage to tissue. The purpose of our study was to investigate that hydrogen (H2) gas has potential as an antioxidant in preventive and therapeutic applications against ischemia - reperfusion injury.
[Methods] We first determined the dose at which cytotoxicity develops in a period of 1 h upon Antimycin A exposure in H9c2 cells through the WST assay. Antimycin A impaired cell viability in a concentration-dependent manner over the tested concentration range (0-500 μM).
[Results] A maximum reduction was 76.5±4 of the control group (at 500 μM of Antimycin A). The H9c2 cells were pretreated with H2 gas for 18 h, after which co-incubated with 500 μM of Antimycin A for additional 1h. Co-treatment of H2 gas relieved reduced viability of Antimycin A-stimulated cells.
[Conclusions] These results suggested the protective effects H2 gas against Antimycin A- induced injury in H9c2 cells.

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