Effects of Euterpe oleracea on learning and memory in adult male rats

[Speaker] Cafer Yildirim:1
[Co-author] Sule Aydin:1, Basak Donertas:1, Setenay Oner:2, Fatma Sultan Kilic:1
1:Department of Pharmacology, Eskisehir Osmangazi University, School of Medicine, Turkey, 2:Department of Biostatistic, Eskisehir Osmangazi University, School of Medicine, Turkey

Background: Euterpe oleracea (EO) contains polyphenolic structures, especially bioactive substances such as phenolics, flavonoids and anthocyanins with different bioactive properties including antioxidant, anti¯inflammatory, and analgesic activities. Age¯related neurodegenerative diseases compromise impairment of memory and learning which are directly linked with increases in oxidative stress. In this study, it has been proposed that EO might attenuate these impairments considering its antioxidant activities.
Materials: Male Wistar rats (n= 72, 250±25g) were used and divided into the following groups: Control (saline); EO 100 and 300 mg/kg; scopolamine 1.5 mg/kg; mecamylamine 7.5 mg/kg; scopolamine 1.5 mg/kg combinations with EO 100, 300 mg/kg and rivastigmine 1.5 mg/kg; EO 100 mg/kg+mecamylamine 7.5 mg/kg. Saline, EO and rivastigmine were administered via gastric lavage during 5 days and on the 6th day 30 minutes after intraperitoneal scopolamine and mecamylamine injections. Locomotor activity and Morris Water Maze test were performed 90 minutes after drug administrations. To determine the acetylcholine levels, hippocampus were isolated at the end of the experiments. One Way and Two Way ANOVA were used for statistical analyses and p<0.05 was considered significant.
Results: EO 100 and 300 mg/kg made no significant alterations on learning and memory compared to control. Mecamylamine and scopolamine increased the latency to escape platform and decreased time spent in escape platform quadrant on memory tests compared to control (p<0.05). Scopolamine combinations of EO 100, 300 and rivastigmine and mecamylamine combination with only EO 100 mg/kg doses were improved memory. There were significant differences between the first and the 5th days on learning tests in all groups (p<0.001), but no differences between the groups. There were no significant differences between the groups in total number of movements in locomotor activity test. Hippocampal acetylcholine levels supported all memory results.
Conclusion: Both doses of EO made no alteration on learning and memory but it improved nicotinic and muscarinic receptors mediated impaired memory as rivastigmine did.
This study was supported by Eskisehir Osmangazi University Commission of Scientific Researces under the projects number: 2016¯1168.
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