Intravital imaging of renal pathophysiology and pharmacology

[Speaker] Daisuke Nakano:1
1:Department of Pharmacology, Kagawa University, Japan

Kidney is the organ that has one of the most complicated architecture in the body. The sophisticated architecture of nephron made its physiology difficult to unravel and the variety for experimental approach for renal (patho)physiology is limited so far. These factors prevent drug development and widening the pharmacological research. In vivo optical imaging is a long-time used powerful tool to for evaluating the renal physiology of small animals and has the potential to elucidate the unknown regulatory mechanisms of renal pathophysiology. The experimental approach using multiphoton laser microscopy expand what we can do by using intravital imaging and has revealed the heterogeneity of blood flow in each capillary in several experimental models of disease, glomerular sieving of several proteins, slowing of tubular flow in oliguric model, and the balance between the ischemic and normoxic capillary number correlates with renal function. Moreover, we recently found that ischemia/reperfusion-induced damage changed renal tubular glucose handling and that the inhibitor of sodium glucose cotransporter 2 induced remarkable pharmacological effects in this condition. At this symposium, I would like to discuss the recent progress in our understanding of renal (patho)physiology and pharmacology.
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