Hydrogen sulfide promotes skin wound healing via inhibitory of oxidative stress

[Speaker] Mengting Xu:1
[Co-author] Shengju Yang:2, Guoliang Meng:1, Wei Zhang:1
1:School of Pharmacy, Nantong University, China, 2:Affiliated Hospital of Nantong University, China

Background Hydrogen sulfide (H2S) is an important gasotransmitter involved in physiological function. However, the role of H2S on skin wound healing and the detailed mechanisms were not known well.
Methods 129S1/SvImJ mice were intraperitoneally injected with NaHS for 2 weeks. Normal saline (NS) administration served as control. Then a round wound of 6 mm diameter with depth into the dermis was made with biopsy perforator at the back spine. The skin would was photographed and the blood flow was recorded using laser Doppler after operation. The sample from skin trauma were collected at different time points and subjected to Hematoxylin-eosin staining to detect the histopathological changes. The oxidative stress level in the skin was assessed by dihydroethidium (DHE) staining. The expression of dynamin related protein 1 (DRP1) and optic atrophy 1 (OPA1) were measured by immunofluorescence.
Results Compared with the NS control group, NaHS pretreatment significantly reduced the wound area and increased blood flow. The local skin epidermis area and epidermis thickness of the wound were significantly increased. The fluorescence intensity with DHE staining decreased after NaHS pretreatment. NaHS also decreased DRP1 expression but increased OPA1 expression in the skin.
Conclusions Supplementary of H2S accelerated the skin wound healing, which might be related to enhanned local blood flow and attenuated oxidative stress.

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