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PO4-9-18

Evaluation of the effect of ethanolic stem bark extract of Mangifera indica on Hormonal profile and Semen quality of male albino rats

[Speaker] Adeyinka A. Aderinola:1
[Co-author] Jane I. Ejiofor:2
1:Department of Pharmacology, Olabisi Onabanjo university, Ago-iwoye, Nigeria., Nigeria, 2:Department of Pharmacology and Therapeutic, Ahmadu Bello University, Zaria., Nigeria

Background: Medicinal plants are used globally for prevention and treatment of wide range of diseases. It is sometimes used as alternatives and/or adjuvants to modern medicine. However, detailed toxicological evaluation of most herbal plants especially on Reproductive functions has not been fully explored. This research focused on evaluating the effect of ethanolic stem bark extract of Mangifera indica on the Hormonal profile and Semen quality of male albino rats.
Method: The stem bark of Mangifera indica was air-dried, ground, macerated in 90% ethanol and concentrated to dryness in a water bath. The dry extract was then reconstituted using distilled-water. Twenty-four (24) male albino rats weighing between 120-150g each were used for this study. They were divided into 4 groups (A, B, C and D) of six rats per group. Group D served as the control (without test substance) while groups A, B and C were fed with of 250mg/kg, 500mg/kg and 1000mg/kg body weight (BW) of the extract for the period of 20 days and then tested for the hormonal profile and semen quality.
Result: The results obtained from hormonal analysis showed that the extract produced significant (p<0.05) increase in the levels of Luteinizing hormone (LH) and Testosterone at 250mg/kg and also increase in Follicle Stimulating hormone (FSH) level at 1000mg/kg when compared with the control. However, the extract significantly (p<0.05) reduced the levels of Luteinizing hormone (LH) and Testosterone at 500mg/kg and 1000mg/kg while the level of Follicle Stimulating hormone (FSH) was reduced at 250mg/kg and 500mg/kg.
For the Semen analysis, the extract significantly (p<0.05) increased the sperm motility, sperm morphology and sperm count of the rats in a dose dependent manner with more pronounced effect at 1000mg/kg when compared with the control.
Conclusion: Administration of the extract produced an increase in sperm count, sperm motility and sperm morphology of the male albino rats, this may be as a result of the ability of the extract to increase the level of Follicle Stimulating hormone (FSH) which is responsible for spermatogenesis. This suggests that the stem bark of Mangifera indica may be incorporated in the formulation of male fertility drugs.

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