A New Phase of Organoid Culture: EMT, CSC, Exosome, and HSP

[Speaker] Takanori Eguchi:1
1:Dental Pharmacology, Okayama Univ, Japan

Two-dimensional (2D) cell culture system by using plastic wares made innovation and has been often used in biological research, including drug screening; however, the 2D culture system forces malignant transformation and integrin expression on cancer cells. In contrast, cells can form three-dimensional (3D) aggregates, organoids or tumoroids in 3D culture environments. We have examined alteration of cellular and organoid morphologies, intra-tumoral hypoxia levels, epithelial-mesenchymal transition (EMT), gene expression profiles and secretion of extracellular vesicles (EVs) or exosomes by culturing cancer cells in 2D and 3D matrix-free NanoEnvironments (1, 2). One of our studies clarified a novel EMT inhibitor through a novel drug screening system using NanoCulture Plates (1). Our recent study further clarified a novel aspect of tumoroids with properties of cancer stem-like cells (CSCs) that secrete particular EVs and heat shock proteins (2).

1. Arai K, Eguchi T, et al, PLOS ONE, 2016 Sep 13;11(9).
2. Eguchi T, Sogawa C, et al, PLOS ONE, in press.

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