Regenerative potential of Dental Pulp Stem Cells grown on Nano fibrin and Hydrogel based Scaffolds for Odontogenic Differentiation

[Speaker] Harjeet Singh:1
[Co-author] Syed Y Husain:2, Shivangi Sharma:3, Lokendra Sharma:3
1:Dept. of Biochemistry SMS Medical College, India, 2:Dept of Dental Materials, Rajasthan University of Health Sciences, India, 3:Sawai Man Singh Medical College and Hospitals, India

The present study is to evaluate and recognize the various sites of a deciduous tooth and/or impacted third molar that can be regarded as a potential reservoir for stem cells. A deciduous tooth that has exfoliated from a child or impacted third molars from adult donors will be taken for the study after their written consent. With the help of a diamond cutter the tooth will be sectioned to extract dentine, pulp and the periodontal ligament. Out of the sites chosen, dentinal pulp has been proven to be a potential site for stem cells, whereas dentine and periodontal ligament being potential sites do not have much support to contain stem cells. Apical papilla and periodontal ligament are closely related to the blood supply of the tooth and have been known to have regenerative capability. Due to its reparative nature, theoretically these sites have been known to be potential sites of stem cells too. Fibrin has lots of biodegradability, biocompatibility, non-toxicity and of low cost. Gelatin can be used as a valuable biopolymer in tissue engineering. (e.g., wound-dressing and bone scaffolding). Fibrin on the other hand can be prepared from blood sample and used autologously to patients requiring furcation procedures. We aim to evaluate and compare the role of nano-gelatin and Bioengineered Scaffolds on the attachment, proliferation, and osteogenic differentiation of human dental pulp stem cells (DPSCs). The design of novel scaffolding materials will be based on mimicking the architecture of natural dental extracellular matrix which may provide as in vivo environments for proper cell growth. This methodology will involve the combination of nano-fibred gelatin as well as biodegradable hydrogel based tooth scaffold.
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