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PO4-2-40

Morin ameliorates isoproterenol induced myocardial necrosis in rats

[Speaker] Vipin K. Verma:1
[Co-author] Dharamveer Arya:1, Jagriti Bhatia:1
1:Pharmacology, All India Institute of Medical Sciences, India

Objective:
To study the cardiprotective effect of Morin in isoproterenol induced myocardial injury in rats.
Introduction:
Oxidative stress plays a major role in myocardial injury following ischemia. Morin, a bioflavonoid, possesses anti-inflammatory, antioxidant and antiapoptotic properties. Hence, we evaluated the cardioprotective potential of Morin following isoproterenol-induced myocardial injury in rats.
Methods:
The study protocol was approved by the institutional animal ethics committee (03/IAEC-1/2017).Male Wistar rats (n=6 per group) were treated orally with different doses of Morin (20mg/kg, 40mg/kg or 80 mg/kg) +ISO for 28 days. After 26 days, ISO (85mg/kg s.c.) was injected consecutively for two days with an interdose interval of 24 hours to induce myocardial necrosis. On the 29th day, rats were anaesthetized with sodium pentobarbitone (60mg/kg) for the recording of hemodynamic parameters. Also, the rat heart and sera were collected for analysis of morphological and biochemical parameters.
Results:
In ISO-treated rats, there was a decrease in levels of antioxidant enzymes and an increase in the levels of pro-inflammatory cytokines (TNFα IL-β
 and IL-6).Also, myocardial injury markers like creatine kinase MB and lactate dehydrogenase (LDH) were elevated. On immunohistochemistry, Bax/Bcl-2 ratio was found to be increased. Morin dose-dependently improved hemodynamic profile, increased the levels of anti-oxidants and reduced the levels of inflammatory markers as well as apoptosis. Histopathological and ultrastructural studies demonstrated that Morin exerted good cardioprotection. The maximum cardioprotection was observed at the dose of 80mg/kg.
Conclusion:
Morin was shown to exert significant cardioprotection.The beneficial effect of Morin was attributed to its significant antioxidant, anti-inflammatory and antiapoptotic properties.

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