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PO4-1-8

Effects of angiotensin II on excitatory synaptic transmission in rat nucleus tractus solitarius neurons

[Speaker] Yoshiaki Ohi:1
[Co-author] Misaki Tanimoto:1, Daisuke Kodama:1, Akira Haji:1
1:Laboratory of Neuropharmacology, School of Pharmacy, Aichi Gakuin University, Japan

 Baro- and chemoreceptor afferents project to the nucleus tractus solitarius (NTS) neurons and these signal transmission play an important step in cardiorespiratory control. The expression of angiotensin II type 1 (AT1) and 2 (AT2) receptors is confirmed in the NTS. Activation of AT1 receptors is demonstrated to activate endotherial nitric oxide synthase and produced NO stimulates voltage-dependent Ca2+ currents (Cheng et.al., 2012, Li et.al., 2014). On the other hand, there is a report that AT1 receptors inhibit nitric oxide synthesis by NADPH oxidase (Wang et.al., 2006). Therefore the physiological roles of angiotensin II in the NTS are controversial. In this report, we aimed to reveal the effects of angiotensin II on the excitatory synaptic transmission in the rat NTS neurons by using a slice patch-clamp technique.
 Superfusion of angiotensin II (1 μM) increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) in 33 % (6/18) of neurons and decreased it in 39 % (7/18) of neurons without any significant effect on the amplitude. It had no effect in the remaining 28 % (5/18) neurons. The prior application of AT1 receptor antagonist losartan (10 μM) blocked the increase in the sEPSCs frequency induced by angiotensin II and that of AT2 receptor antagonist PD-123319 (30 μM) blocked the decrease in the sEPSCs frequency. The angiotensin II-induced increase in the sEPSCs frequency was also blocked by prior application of L-NAME (100 μM).
 The present study demonstrates that angiotensin II (1) facilitates glutamate release from the presynaptic terminals by production of NO through AT1 receptors in some class of NTS neurons, and (2) inhibits glutamate release from the presynaptic terminal via AT2 receptors in other class of neurons. These results suggest that angiotensin II modulates baro and chemoreceptor reflex through AT1 and AT2 receptor activation in the NTS.

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