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PO3-10-33

Cutleaf Groundcherry Leaf Extract Prevents the Development of Preeclampsia through Inhibition of Oxidative Stress and Antiangiogenic Factor

[Speaker] Dian Nugrahenny:1,2
[Co-author] Setyawati Soeharto:1, Nur Permatasari:1, Elly Mayangsari:1
1:Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Indonesia, 2:Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Indonesia

Background: Preeclampsia is the major cause of maternal and fetal morbidity and mortality in the world. The oxidative stress and imbalance of angiogenic-antiangiogenic factors contribute to the development of the disease. This study aimed to evaluate the effect of cutleaf groundcherry (Physalis angulata L.) leaf methanolic extract on the prevention of preeclampsia development on L-NG-nitroarginine methyl ester (L-NAME)-induced pregnant Wistar rats and to identify the mechanism behind the effect.

Methods: Twenty-five pregnant Wistar rats were randomly divided into five groups as follow. Normal rats received phosphate-buffered saline (PBS) injection on G5-G17, preeclampsia rats injected with L-NAME (40 mg/kgBW s.c.) on G5-G17, and three groups of preeclampsia rats orally supplemented with cutleaf groundcherry leaf methanolic extract at doses of 90, 270, or 450 mg/kgBW/day on G1-G17. The maternal blood pressures were measured using a tail-cuff method on G4, G8, and G17. The rats were sacrificed on G18. Serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels were measured by colorimetry. Serum nitrite/nitrate levels were determined using Griess reaction colorimetry. Serum soluble fms-like tyrosine kinase 1 (sFlt-1) levels were evaluated using enzyme-linked immunosorbent assay (ELISA). All fetuses were weighed on an analytical balance.

Results: The L-NAME injection on G5-G17 significantly (p < 0.05) increased systolic and diastolic blood pressures, increased serum levels of MDA and sFlt-1, also decreased serum SOD activity and nitrite/nitrate levels, as well as reduced fetal weight in pregnant rats compared with the normal pregnant group. The administration of cutleaf groundcherry leaf extract on G1-G17 significantly (p < 0.05) prevented the increase in systolic and diastolic blood pressures, inhibited the increase in serum levels of MDA and sFlt-1, also inhibited the reduction in serum SOD activity and nitrite/nitrate levels, as well as prevented the reduction in fetal weight in L-NAME-treated pregnant rats compared with the normal pregnant group. The effective dose of cutleaf groundcherry leaf extract was 90 mg/kgBW/day.

Conclusion: The cutleaf groundcherry leaf extract suppresses the oxidative stress and antiangiogenic factor. Thus, it is beneficial as prevention of the development of preeclampsia in both mother and fetus.
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