Program

PO3-10-31

Protective effect of Stachytarpheta jamaicensis (L.) Vahl extract against hydrogen peroxide-induced oxidative stress in human vascular endothelial cells

[Speaker] Pearl Majorie P. M. Liew:1
[Co-author] Yoke Keog Y. K. Yong:1, Hasnah H. Bahari:1, Zuraini Z. Ahmad:2
1:Human Anatomy Department, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia, 2:Biomedical Sciences Department, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia

Purpose: Stachytarpheta jamaicensis (L.) Vahl has long been used in traditional medicines, but its role in the protection of endothelial cells from ROS damage has not yet been established. This study aimed to investigate protective effect of an ethyl acetate extract of Stachytarpheta jamaicensis (L.) Vahl (EASJ) on human umbilical vein endothelial cells (HUVECs) under oxidative stress induced by H2O2.

Methods: HUVECs were pre-treated with EASJ prior to H2O2 stimulation. Cell viability was evaluated by 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Endothelial permeability assay was examined using fluorescein isothiocyanate (FITC)-dextran. ROS generation in H2O2-stimulated HUVECs was evaluated by the fluorescence intensity of 2',7'-Dichlorofluorescin diacetate (DCFH-DA). Additionally, the effect of EASJ on F-actin rearrangement and VE-cadherin distribution were assessed using immunofluorescence staining.

Result(s): The EASJ concentrations at 10, 50 and 75 ug/mL yielded cell viability higher than 80%. Pre-treatment with EASJ at 75 ug/mL demonstrated anti-inflammatory activity through suppression of endothelial hyperpermeability to 89.12 % of control and attenuated the excessive ROS production to 73.73%. Using an image analyser, reduced formation of stress fibers in actin cytoskeleton and tightening of VE-cadherin in H2O2-induced HUVECs were also observed.

Conclusion(s): These findings show that EASJ at 75 ug/mL has a protective effect on HUVECs against hydrogen peroxide-induced damage. This approach may serve as an alternative for the treatment of vascular diseases associated with oxidative stress.


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