Miswak sticks extract exerts anti-inflammatory properties in carrageenan test in rats

[Speaker] Vahid Nikoui:1
[Co-author] Saeed Mehrzadi:1, Azam Bakhtiarian:2,3, Mohammad Yahya Karimi:1, Mehrdad Foroohandeh:4, Vida Nikouei:4, Roya Fallah:5, Amir Rashidian:2, Muhammad Imran Khan:6
1:Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran, 2:Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran, 3:Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran, 4:Department of Oral Pathology, Dental School, Isfahan University of Medical Science, Isfahan, Iran, 5:Department of Cardiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran, 6:Department of Pharmacy, Kohat University of Science and Technology, Kohat, KPK, Pakistan

Background: Miswak is the product of Salvadora persica L. that has antibacterial, antifungal antiplasmudium, anticonvulsant, and gastroprotective properties. In this study, the anti-inflammatory effect of aqueous alcoholic crude extract of miswak sticks (Salvadora persica L.) was investigated in carrageenan-induced rat paw edema.
Methods: Adult male Sprague dawley rats were divided into five groups. Group 1 received normal saline. Groups 2 to 4 received crude aqueous alcoholic extract of Salvadora persica L. at dose of 75 to 100 mg/kg. Group 5 received indomethacin (5 mg/kg). All injections were given intraperitoneally. After 30 minutes, carrageenan was injected into plantar surface of paw, and edema was measured using a micrometer. For multiple comparisons, one-way ANOVA followed by Tukey`s post-hoc test was carried out. P-values less than 0.05 were considered statistically significant.
Results: Indomethacin decreased edema, significantly compared to saline-injected group (P<0.0001). Salvadora persica L. extract at dose of 50 mg/kg reduced inflammation on 4 and 5 hours after carrageenan injection (P<0.05). Doses of 75 mg/kg (P<0.01) and 100 mg/kg (P<0.001) of this extract exerted anti-inflammatory effect at 4, 5, and 6 hours after carrageenan injection.
Conclusions: Our data revealed that Salvadora persica L. extract possesses anti-inflammatory properties, and further experiments on the possible use of this plant in inflammatory disorders are required.

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