Time-Kill Assay and Post-Antibiotic Effect of Acetone Extract from the Twig of C. odontophylum against Methicillin-Resistant Staphylococcus Aureus (MRSA) Mu50 Strain

[Speaker] Dayang F Basri:1
[Co-author] Nur A Yahya:1
1:School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Malaysia

Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that contributes to hospital (HA-MRSA) and community (CA-MRSA) infection worldwide. Vancomycin has been the therapeutic drug of choice against MRSA infection which slowly develop resistance. This lead to research on natural sources as an alternative treatment. Canarium odontophyllum (dabai) is one of the famous indigenous fruits in Sarawak Malaysia. High amount of tannin in the twig of C. odontophyllum was suggested to be the bioactive phytoconstituent responsible for antimicrobial activity. The current study aimed to investigate time-kill assay (TKA) and post-antibiotic effect (PAE) of acetone extract from the twig of C. odontophyllum against MRSA Mu50 strain. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of extract and vancomycin against MRSA were determined by broth microdilution technique and streak plate method. TKA of extract against MRSA was determined at 1XMIC, 2XMIC, 4XMIC and 8X MIC whereas for vancomycin, 1XMIC was used. PAE of extract and vancomycin against MRSA was assessed at 10XMIC. The extract exhibited bacteriostatic action against MRSA with MIC and MBC values of 1.563 mg/ml and 3.125 mg/ml respectively. From TKA analysis, the extract at 8XMIC showed bactericidal activity at 16 hr compared to vancomycin at 24 hr. The PAE time of the extract was 3.6 0.51 hr against MRSA compared to vancomycin at 2.4 0.68 hr. These findings suggested that the twig from C. odontophyllum have the potential to be developed as antimicrobial agent against MRSA as it displayed bactericidal effect at 8X MIC and prolonged PAE time.
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