Post-translational Modification of GRASP Is a Essential for Unconventional Secretion of CFTR

[Speaker] Jiyoon Kim:1
[Co-author] Min-Goo Lee:1
1:Pharmacology, Yonsei University College of Medicine, Korea

Induction of endoplasmic reticulum (ER)-to-Golgi blockade or ER stress induces Golgi reassembly stacking protein (GRASP)-mediated, Golgi-independent unconventional cell-surface trafficking of the folding-deficient delta F508-cystic fibrosis transmembrane conductance regulator (CFTR). However, molecular mechanisms underlying this process remain elusive. Here, we show that phosphorylation-dependent dissociation of GRASP homotypic complexes and subsequent relocalization of GRASP to the ER play a critical role in the unconventional secretion of CFTR. Immunolocalization analyses of mammalian cells revealed that the Golgi protein GRASP55 was redistributed to the ER by stimuli that induce unconventional secretion of delta F508-CFTR, such as induction of ER-to-Golgi blockade by the Arf1 mutant. Notably, the same stimuli also induced phosphorylation of regions near the C-terminus of GRASP55 and dissociation of GRASP homomultimer complexes. Furthermore, phosphorylation-mimicking mutations of GRASP55 induced the monomerization and ER relocalization of GRASP55, and these changes were nullified by phosphorylation-inhibiting mutations. Strikingly, the inhibition of phosphoinositide 3-kinases class 3 (PI3KC3) blocked the monomerization of GRASP55. These results provide mechanistic insights into how the phosphorylation activates the unconvetional function of GRASP55 and mediates the ER stress-induced unconventional secretion pathway.
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