Psoralidin promotes osteogenesis and inhibits adipogenesis via different estrogen receptor signalings

[Speaker] Xinluan Wang:1
1:Translational Medicine R&D Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, China

Objective: This study aimed to investigate the molecular mechanism of psoralidin on promoting osteogenesis and inhibiting adipogenesis.
Methods: Preosteoblasts MC3TC-E1 cells and preadipocyte 3T3-L1 cells were used in this study. ICI 182,780 (ICI) as a specific antagonist of estrogen receptor (ER) was used to block ER signaling in these two cell lines. Alizarin Red S and Oil Red O staining were used to evaluate the effects of psoralidin on promoting mineralization and inhibiting oil droplets accumulation, respectively. During differentiation processes, the osteogenic- or adipogenesis-related markers were detected.
Results: Psoralidin could enhance calcium nodule formation through up-graduating alkaline phosphatase activity and osteocalcin level. As to adipogenesis, psoralidin decreased adipocytes formation and accumulation through declining peroxisome proliferator activated receptor gamma, CCAAT-enhancer-binding protein alpha, lipoprotein lipase and fatty acid binding protein 4 mRNA levels. Meanwhile, we found fat-related factors adiponectin, resistin and leptin levels were all decreased with psoralidin treatment. ICI could completely block the effect of psoralidin on promoting calcium nodule formation, but only contribute partial effect on inhibiting adipogenesis. G-15 (a selective G protein-coupled receptor 30 antagonist) and ICI had similar efficiency on blocking the effect of psoralidin on inhibiting adipogenesis, yet G-1(a selective G protein-coupled receptor 30 agonist) could not affect the function of psoralidin on promoting mineralization.
Conclusion: Psoralidin exhibited similar efficiency and mechanism with estradiol on promoting osteogenesis and inhibiting adipogenesis. Meanwhile, psoralidin promoted maturation and mineralization of MC3T3-E1 cells via mediating ER related classical genomic signaling events, while psoralidin inhibited adipocyte differentiation and maturation of 3T3-L1 cells via rapid cellular signaling events of estrogen receptor.
Acknowledgements: This work was supported by National Nature Scicence Foundation of China (81773964).

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