Program

PO2-12-24

Effect of ABP on cognitive impairment in ovariectomized mice model

[Speaker] Ariyawan Tantipongpiradet:1
[Co-author] Orawan Monthakantirat:2, Kinzo Matsumoto:3, Suresh Awale:3, Dya Fita Dibwe:3, Chantana Boonyarat:2, Supawadee Daodee:2, Natdanai Musigavong:4, Supaporn Pitiporn:4, Yaowared Chulikhit:2
1:Graduate school, Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand, 2:Departments of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand, 3:Institute of Natural Medicine, University of Toyama, Japan, 4:Chao Phya Abhaibhubejhr Hospital, Thailand

Cognitive impairment is a neurological symptom caused by the imbalance of an estrogen level in menopause women. ABP (Abhaibhubejhr herbal formula capsule for menopause), a traditional medicine consisting of 22 different herbs, has long been used for menopausal symptoms in Thailand. In the present study, we investigated the effects of ABP on cognitive deficits and its underlying mechanisms of action in ovariectomized (OVX) female mice, an animal model of menopause. OVX mice showed not only cognitive deficits, which were elucidated by the Y-maze, Morris water maze and novel object recognition tests, but also the down-regulated expression of genes encoding estrogen receptors (ER)(ER-alpha and -beta). This model also had an increased level of hippocampal lipid peroxidation. OVX-induced behavioral and neurochemical alterations were attenuated by daily treatment with ABP and 17beta-estradiol. Since imbalance between oxidant and antioxidant systems is reportedly implicated in cognitive deficits, we analyzed antioxidant activities of ABP in vitro using radical scavenging assays 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, ABP showed radical scavenging activity with IC50 58.59 and 44.99 ug/ml, respectively. Moreover, HPLC analysis revealed that ABP contained antioxidant constituents such as gallic acid, kaemferol, and rutin. These results suggest that ABP exerts the ameliorative effects on OVX-induced cognitive deficits in part via suppression of OVX-induced oxidative stress in the brain. Therefore, ABP may offer an alternative choice as a therapeutic medicine for cognitive deficits observed in the menopausal transition.
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