Program

PO2-12-19

Metabonomics reveals the effect of Sailuotong capsule on brain homogenate in rat model with multiple cerebral infarctions

[Speaker] Mingqian Sun:1
[Co-author] Yehao Zhang:1, Cong Zhang:1, Lan Miao:1, Li Lin:1, Jianxun Liu:1
1:Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, China

Background: Vascular dementia (VD) is an acquired intellectual impairment due to impairment in brain function caused by cerebrovascular disease, such as stroke. Traditional Chinese Medicine (TCM) formula is a herb combination and could impact multiple targets and induce synergistic therapeutic effects which may provide new directions for the treatment of VD. Sailuotong capsule (SLT) is a new standardised three-herb formula that combines with extracts of Panax ginseng, Ginkgo biloba and Crocus sativus. It has been demonstrated to improve cognition of VD by the reports of animal experiments and preliminary clinical trial. This work aims to explore the action mechanism of SLT on the metabolic response of rat model with multiple cerebral infarctions (MCI).
Methods: The detection of metabolites related to SLT intervention of MCI rats in brain homogenates was performed by liquid chromatography coupled to time of flight mass spectrometry (LC-TOF-MS). Partial least-squares to latent structure-discriminant analysis (PLS-DA) was used for multivariate statistical analysis, and MetaboAnalyst was performed to illustrate the metabolic pathways of therapeutic action of SLT. TTE staining, electron microscopy and neurological detection were performed in the assessment the effect of the SLT in MCI rats.
Resluts: SLT treatment attenuates the neurological dysfunction and reduces the brain lesion volume in MCI rats. It also could reverse the abnormal metabolites (arginine, N-acetylaspartate, histidine, malic acid, and glutathione, etc.) in brain to normal level. These potential biomarkers for efficacy of SLT mainly involved in urea cycle, oxidative stress, citrate cycle, aspartate and glutamate metabolism and histidine metabolism.
Conclusion: The study reveals the potential biomarkers and metabolic mechanism of neuroprotection effect of SLT in MCI rat model. It also indicates this metabonomic approach is useful to study mechanism of therapeutic effects of TCM.
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