Effects of D-limonene on neurotropic factors and ionic currents in the olfactory nerve cells Rolf B1. T

[Speaker] Yi-Ching Lo:1
[Co-author] Sheng-Nan Wu:2, Wan-Hsuan Chang:1, Yu-Ting Tseng:1, Horng-Huey Ko:3
1:Department of Pharmacology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, 2:Department of Physiology, National Cheng Kung University Medical College, Taiwan, 3:Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Taiwan

Background: D-limonene (D-LIM) is the main component of essential oil from Citrus aurantium L. peel, which is commonly used as an alternative treatment for insomnia, anxiety and epilepsy. In this study, we investigated the effects of D-LIM on neurotropic factors and ion currents in the olfactory nerve cells Rolf B1.T.
Methods: Recordings of membrane potential or ion currents were measured with glass pipettes in the whole-cell configuration of the patch-clamp technique. Cell viability was determined by MTT and LDH assays. Protein expressions were analyzed by western blots. Reactive oxygen species production was measured by flow cytometer.
Results: In Rolf B1.T cells, D-LIM directly stimulated BDNF-related neurotrophic pathway. In 1-methyl-4-phenylpyridine ion-treated Rolf B1.T cells, D-LIM increased cell viability, attenuated ROS production and enhanced BDNF-related signaling. Moreover, D-LIM suppressed the transient and late components of voltage-gated Na+ current and sustained K+ current in Rolf B1.T cells.
Conclusions: Our data demonstrated that D-LIM possesses neuroprotective effects via enhancing neurotropic pathway, attenuating oxidative stress and modulating ion currents.

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