Short-term stability study of acyclovir-loaded solid lipid nanoparticles

[Speaker] Haniza Hassan:1
[Co-author] Siti Khadijah Adam:1, Ahmad Fuad Shamsuddin:2, Rusliza Basir:1
1:Dept of Human Anatomy, Faculty of Medicine and Health Sciences, UPM, Serdang, Selangor, Malaysia, 2:Faculty of Pharmacy & Health Sciences, UniKL-RCMP, Ipoh, PERAK, Malaysia

Introduction. Poor oral bioavailability of acyclovir has become one of the major drug delivery concerns globally. Nanoparticulate system have been explored and exploited to overcome current pharmacokinetic limitations of acyclovir. Nanoparticles offer many great advantages including physicochemical stability and sustained release properties. Shelf-life stability test is one of the important measures of successful development of a drug carrier and pre-requisite for future clinical trials of a pharmaceutical product.
Aim. In this study, we aimed to observe the physicochemical stability of solid lipid nanoparticles suspensions loaded with acyclovir.
Methods. Two different types of solid lipid nanoparticles formulations were evaluated in this study; glyceryl palmitostearate (Biogapress Vegetal BM 297 ATO) and glyceryl behanate (Compritol 888 ATO), with polysorbate 80 (Tween 80) acted as emulsifying agent. After preparation of the acyclovir-loaded solid lipid nanoparticles based on the optimized formulations, the nanoparticles suspensions were stored at 4, 25 and 40 degree celcius for a period of three months. All formulations were subjected to morphological tests such as size, polydispersity and zeta potential measurements.
Results. All solid lipid nanoparticles containing acyclovir prepared from glyceryl behanate and glyceryl palmitostearate stored at 4 degree celcius were considered stable throughout the three months of testing period as no significant difference of particle size, zeta potential and polydispersity index were observed.
Discussion. Excellent particle's stabilization was achieved could be due to presence of free emulsifier in the suspension that prevent particle collision during storage at low temperature.
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