Program

PO1-13-21

Involvement of secernin1 in mouse development

[Speaker] Yu Tezuka:1
[Co-author] Kohei Ishigaki:1, Hikaru Otsu:1, Shintaro Takahashi:1, Yuumi Kikuchi:1, Ryouta Hiramatsu:1, Atsushi Sanbe:1
1:Pharmacotherapeutics, Iwate Medical University, Japan

[Introduction]
Secernin 1 (SCRN1) is a member of the secernin family, and is reported to be a tumor-associated antigen. The functional role of SCRN1 during development remains uncertain. In this study, we examined the effects of the loss of SCRN1 function in mice development.
[Methods]
To address the functional role of SCRN1 in mice that are heterozygous for a gene trap mutation of SCRN1 (SCRN1GT/+) and mice that are homozygous for a gene trap mutation of SCRN1 (SCRN1GT/GT), mice were generated on a C57BL/6Cr Slc genetic background and phenotype was analyzed.

[Results]
The litter size resulting from breeding male SCRN1GT/+ and female SCRN1GT/+ mice was smaller than that resulting from breeding wild-type mice (SCRN+/+). The birth ratio of SCRN1GT/GT mice was markedly reduced (to approximately 9%) by breeding SCRN1GT/+ mice. Furthermore, the body size of SCRN1GT/GT mice was lower than that of SCRN1GT/+ and SCRN+/+ mice at birth and at 4 weeks of age. The birth ratio of SCRN1GT/GT mice was approximately 22% at 10 days post coitum. No significant differences in litter size or embryonic size were observed between litters from the SCRN+/+ mouse mating and those from the SCRN1GT/+ mouse mating at 10 days post coitum. The birth ratio of SCRN1GT/GT mice and the embryonic size was, however, smaller than that for SCRN1GT/+ and SCRN+/+ mice at 17.5 days post coitum.
[Conclusion]
These data suggest that SCRN1 plays an important role in normal mouse development before birth.

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