Protective effects of the water extract of Liuwei dihuang on methylglyoxal-induced atrophy in myotubes

[Speaker] Yu-Ting Tseng:1
[Co-author] Yi-Ching Lo:1, Chi-Ming Liu:2, Hung-Te Hsu:3,4, Pao-Chu Wu:5
1:Department of Pharmacology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, 2:Department of Nursing, Tzu Hui Institute of Technology, Pingtung, Taiwan, 3:Faculty of anesthesiology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, 4:Department of Anesthesia, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan, 5:School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

Background: The Chinese herbal formula Liuwei dihuang (LWDH), composed of dihuang (Rehmannia glutinosa), shanyao (Dioscorea opposite), shanzhuyu (Cornus officinalis), zexie (Alisma orientalis), hoelen (Poria cocos) and mudanpi (Paeonia suffruticosa), is a widely used traditional Chinese medicine for neurosis, diabetes, and osteoporosis. Our previous study demonstrated that the water extract of LWDH (LWDH-WE) protects dopaminergic neurons against Parkinson's toxin and improves motor activity of mice with MPTP-induced Parkinson's disease. Methylglyoxal (MG) is a reactive dicarbonyl intermediate and a precursor of advanced glycation end products. In this study, we investigated the effects of LWDH-WE on MG-induced myotube atrophy in C2C12 cells.
Methods: Cell viability was determined by MTT and LDH assays. Protein expressions were analyzed by western blots. Morphological changes were observed by inverted microscope. Mitochondria membrane potential and reactive oxygen species (ROS) production were measured by flow cytometer. C2C12 myotubes were pre-treated with LWDH-WE for 1 h before MG treatment.
Results: In MG-treated C2C12 myotubes, LWDH-WE increased cell viability and mitochondria membrane potential. LWDH-WE also attenuated MG-induced ROS production. Moreover, LWDH-WE regulated protein synthesis/degradation-related pathways and attenuated MG-induced reduction of myotube diameter.
Conclusions: Our data demonstrate that LWDH-WE possesses potential muscle protective effects against MG-induced toxicity.
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