Program

PO1-9-32

Phytochemistry and Toxicity of Crude Water Soluble Extract of Tradescantia fluminensis in Wistar Rats

[Speaker] Kanyarat Boonprasert:1,2
[Co-author] Tullayakorn Plengsuriyakarn:1,2, Ngampuk Tayana:3, Somboon Kiettinun:4, Kesara Na-Bangchang:1,2
1:Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Thailand, 2:Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Thailand, 3:Drug Discovery and Development Center, Thammasat University, Thailand, 4:Graduate Program in Integrative Medicine, Chulabhorn International College of Medicine, Thammasat University, Thailand

Introduction: Tradescantia fluminensis (TF) has been used as an alternative treatment in Thai traditional medicine for renal failure. However, information on its toxicity and phytochemical has not been investigated.
Aims: To (i) determine phytochemical constituent of the crude water soluble extract from T. fluminensis, (ii) determine the acute and subacute (repeated dose 28-day) toxicity of crude water soluble extract from T. fluminensis in Wistar rats.
Method: For acute toxicity, the extract was administered orally at the highest starting dose level (5,000 mg/kg body weight) and signs of toxicity were observed during the first 30 minutes, periodically during first 24 hours, and then daily for 14 days. For subacute toxicity, the extract was given at the daily dose of 1,000 mg/kg body weight for 28 days and signs of toxicity were observed daily. For phytochemical screening, phenols, flavonoids, anthocyanins, triterpenes/steroids, alkaloids, anthraquinones, coumarin, saponins, quinone, tannins, sugar, proteins and amino acid, glycosides, fat and essential oil, and phloroglucinol contents in extract were investigated. The color intensity or the precipitate formation was used as analytical responses to these tests.
Results: Crude water soluble extract from T. fluminensis exerts no significant sign of toxicity (physiology and laboratory parameters) and none of the animals died during the observation period. The extract contained phenol, tannins and saponins as the major bioactive constituent.
Conclusion: The results of study have suggested there was no obvious toxicity observed with the treatment of crude water soluble extract from T. fluminensis. Further study will be performed to confirm its clinical efficacy in the treatment of renal failure in animal model.

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