Stability of secondary metabolite-producing aquatic endophytic fungi from Lotus against Methicillin-resistant Staphylococus aureus

[Speaker] Surachai Techaoei:1
[Co-author] Umpava Pinruan:2, Salilaporn Nuankaew:2
1:Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Thailand, 2:National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Luang Pathum Thani, Thailand

The research was to conduct the stability potential of secondary metabolite of five aquatic endophytic fungi isolated from Lotus in Thailand, Alternaria longipes, Pleosporales sp., Setosphaeria monoceras, Curvularia lunata and Alternaria eichhorniae. All fungal were cultured in PDA and extract with organic solvent as hexane, ethyl acetate and methanol before tested by using disc diffusion method. The thermal and UV radiation were tested stability for antimicrobial potential. Result showed that the crude extract of Alternaria longipes and Alternaria eichhorniae (ethyl acetate extracts) showed thermal stability at 55-121°C and UV stability with tested microorganism as Methicillin-resistant Staphylococus aureus (MRSA) at inhibition zone between of 10.00±0.00 mm and 10.33±0.57 mm, respectively. The further study will be focused on chemical characterization and function of antifungal activity to apply as natural drugs in the future.
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