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PO1-9-14

8-Gingerol mediates its anti-tumor activities in colorectal cancer cells through inducing apoptosis and cell cycle arrest

[Speaker] Xingwang Zhou:1
[Co-author] Sumin Hu:1, Zhaoshou Yang:1, Wanqin Liao:1
1:Department of Biochemistry and Molecular Biology, Sun Yat-Sen University Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China

Background: Ginger has various pharmacological properties, such as anti-oxidant, anti-inflammatory, anti-cancer activities; however the function and mechanisms of 8-gingerol in colorectal cancer (CRC) remains unknown.
Methods: The cytotoxicity of 8-gingerol in CRC cells was evaluated by MTT assay and clonogenic assay. The migration and invasion abilities of CRC cells were determined by migration and invasion assays. Apoptosis was analyzed using annexin V and PI double staining. Cell cycle progression was analysis by flow cytometry. Protein levels were determined by Western blotting.
Results: We evaluated the action of 8-gingerol in CRC cell lines HCT116 and HT29. MTT assay showed that 8-gingerol exhibited cytotoxicity in CRC cells in both dose-dependent and time-dependent manners. Clonogenic assay also showed that treatment with 8-gingerol significantly decreased colony formation ability of CRC cells. Moreover, 8-gingerol exposure markedly reduced the migration and invasion abilities of CRC cells. Furthermore, annexin V and PI double staining showed that treatment with 8-gingerol induced CRC apoptosis. Flow cytometric analysis showed that 8-gingerol exposure resulted in cell cycle arrest in CRC cells and the levels of cell cycle-related proteins were changed according.
Conclusion: Collectively, our findings demonstrate that 8-gingerol has cytotoxic effects on CRC cell growth and progression, and might serve as a potential therapeutic agent for CRC treatment (This work was supported by the Science and Technology Planning Project of Guangdong Province, 2017A020215170).

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